Real time detection of DNA*RNA hybridization in living cells

Real time detection of DNA.RNA hybridization in living cells.

Demonstrating hybridization between an antisense oligodeoxynucleotide and its mRNA target has proven to be extremely difficult in living cells. To address this fundamental problem in antisense research, we synthesized "molecular beacon" (MB) reporter oligodeoxynucleotides with matched fluorescent donor and acceptor chromophores on their 5' and 3' ends. In the absence of a complementary nucleic ...

Real-Time Interference Detection in Tracking Loop of GPS Receiver

Global Positioning System (GPS) spoofing could pose a major threat for GPS navigation ‎systems, so the GPS users have to gain a better understanding of the broader implications of ‎GPS.‎ In this paper, a plenary anti-spoofing approach based on correlation is proposed to distinguish spoofing effects. The suggested ‎method can be easily implemented in tracking loop of GPS receiver...

Spontaneous Mutation: Real-Time in Living Cells

A new study provides the first direct visualization of DNA replication errors as they become mutations in living cells. After decades of post-hoc inference of mutation rates and mechanisms, a new method opens the possibility of cell-, tissue- and cancer-clone-specific mutation-rate detection and real-time visualization of transient hypermutable states in situ.

Watching real-time endocytosis in living cells

The precise sequence of events promoting clathrin-coated vesicle assembly is still debated. In this issue, Kadlecova et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201608071) test structural models using quantitative microscopy in living cells to investigate the hierarchy and temporal importance of molecular events required for clathrin-coated pit initiation.

Detection of Congenital Mullerian Anomalies Using Real-Time 3D Sonography